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KMID : 0350519950480010147
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Journal of Catholic Medical College
1995 Volume.48 No. 1 p.147 ~ p.156
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The Effects of Temperature and Preservation Solution on Lung Preservation in Ex Vivo Rabbit Model Used for Functional Assessment
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Abstract
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In recent years, both single and double lung transplantation have been introduced as therapeutic options for patients with end-stage lung disease. Unfortunately wide application of these procedures is limited by scarcity of donor organs. A major
cause
of this shortage is the lack of a clinically accepted preservation method for the lung, which hampers distant organ procurement.
We studied about the effects of temperature and preservation solution on the preservation of lungwith ex vivo rabbit lung preservation model recomended by Toronto group. A total of 50 rabbit lungs were studied. A control group and three
temperature
groups, each temperature group had three different preservation solution ; extracellular solution(pow potassium solution), 0.9% isotonic saline, intracellular solution(modified Euro-Collins solution), were evaluated. In control group, the lungs
were
assessed immediately after being harvested. In the preservation groups, the lungs were perfused with preservation solution, kept partially expanded with room air, stored at the predetermined temperature by preservation solution immersion and
later
assessed after 12 hours from the harvesting.
@ES The results were as follows.
@EN 1) The results of preservation at 4℃ were partial oxygen tension 75.2±20.2 mmHg, partial carbon dioxide tension 39.69±6.3 mmHg, wet/dry weight ratio 6.91±0.94, and the results at 10℃ were 91.6±20.1 mmHg, 35.1±5.3 mmHg, 6.45±0.85, and
the
results at 25℃ were 37.9±10.2 mmHg, 53.80±8.4 mmHg, 7.83±0.48.
2) The results of preservation with extracellular solution were partial oxygen tension 88.4±29.7 mmHg, partial carbon dioxide tension 35.78±6.5 mmHg, wet/dry weight ratio 6.22±1.04, and the results with 0.9% isotonic saline were 52.2±18.2
mmHg,
48.81±9.4 mmHg, 7.57±0.52, and the results with intracellular preservation solution were 65.8±24.9 mmHg, 44.21±10.7 mmHg, 7.40±0.58.
3) When the preservation was done at 4℃, the paritial oxygen tesion were 100.252±6.4 mmHg with extracellular solution, and 78.14±2.7 mmHg with intracellular solution, and at 10℃, the partial oxygen tension were 115.24±6.9 mmHg with
extracellular
solution, and 89.04±5.1 mmHg with intracellular solution, and at 25℃. The partial oxygen tension were 49.50±4.2 mmHg with extracellular solution, and 33.14±6.1 mmHg with intracellular solution.
The best results of ex vivo rabbit lung preservation were obtained at 10℃ with extracellular solution as 115.24±6.9 mmHg of partial oxygen tension, 30.36±2.4 mmHg of partial carbon dioxide tension, 5.50±0.47 wet/dry weight ration.
As a result, maintenance of preservation temperature at 10℃ in extracellular solution might be superior than 4℃ and 25℃ with intracellular solution in the preservation of ex vivo rabbit lung for 12 hours.
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